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A Text Book of Biotechnology (with Practical) Sem.-4

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Unit-I.Fundamentals of Microscopy:1.History of Contributors of Microbiology; 2.Microscopy; 3.Outlines of Classification of Microorganisms; 4.Structure and General Characteristics of Bacteria an Vir..

Tags: a text book of biotechnology (with practicals) sem.-4

Unit-I.Fundamentals of Microscopy:1.History of Contributors of Microbiology; 2.Microscopy; 3.Outlines of Classification of Microorganisms; 4.Structure and General Characteristics of Bacteria an Virus; 5.Diseases Causing Pathogens and Symptoms; 6.Structure and General Characteristics of Microalgae and Fungi; Exercise

Unit-II.Culture and Identification of Microorganisms:1.Sterilization; 2.Bacteriological Media; 3.Staining Techniques; 4.Bacterial Growth Curve and Factors affecting Bacterial Growth; 5.Identification of Virus; 6.Algal Media; 7.Fungal Media; Exercise

Unit-III.Basics of Immunology:1.Immunity; 2.Cells of Immune System; 3.Primary Organs of Immune System; 4.Secondary Organs of Immune System; 5.Antigens; 6.Haptens and Types of Adjuvants; Exercise

Unit-IV.Humoral and Cell Mediated Immunity:1.Structue of Immunoglobulin; 2.Monoclonal Antibodies; 3.Major Histocompatibility Complex (MHC); 4.Cell Mediated Immunity; 5.Hypersensitivity; 6.Autoimmunity; Exercise

Practicals: 1.Sterilization Techniques, 2.Preparation of Microbiological Culture Media, 3.Isolation of microorganisms by Pour, spread and streak plate methods, 4.Isolation of Bacteria from Soil, 5.Simple and differential staining (gram’s staining), 6.Bacterial Growth Curve, 7.Microhaemagglutination (ABO & Rh blood grouping), 8.Using Typan Blue to Measure Cell Concentration and viability, 9.Differential Leukocyte Count, 10.Single Radial immunodiffusion technique for the quantitative analysis of the given antige, 11.Enzyme-linked Immunosorbent Assay (ELISA)

Stream
Stream B.Sc.(Biotechnology)-IInd Year, Semester-IVth
Author
Author Dr. Chand Pasha Dr. A. Madhuri Ms. Ingelela Marian Rajini
ISBN
ISBN 978-93-8700-714-7
Pages
Pages 8+356

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